The CAMAG UV Lamp 4 is designed for use in a TLC laboratory.
Two types of UV light are required for inspecting thin-Layer chromatograms:
Long-wave UV light 366 nm
Under long-wave UV light substances that can be excited to fluoresce appear as bright spots, often differently colored, on a dark background. The sensitivity of this detection method increases with the intensity of the UV light and also as more visible light is eliminated.
Short-wave UV light 254nm
Under 254 nm UV substances absorbing at that wavelength become visible, provided the TLC layer contains a fluorescent indicator, e.g. F254. These substances appear as dark spots on a bright background.
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